Biosynthesis of tetracycline by 5-hydroxy-tetracycline-producing cultures of Streptomyces rimosus.

Figure 1. Spray gun used in electron microscopy. A. Assembled spray gun. B. Outer envelope. C. Insert tube. The component parts have the following specifications: 1. Nozzle head X inch threads, 0.030 inch aperture. 2. "O" ring, Y8 inch outside diameter (o.d.) and Y inch inside diameter (i.d.). S. X8 inch threads, tapered end. 4. Brass tubing %6 inch o.d.; 0.035 inch wall. .946 inch threads, movable; C14 threads over B5. 6. Flanged end, Y inch diameter; 342 inch long. 7. Side outlet for hose connection Y4 inch diameter and 132 inch long, 300 angle. 8. Modified 27 gauge needle, 0.006 inch aperture. 9. M inch solid round brass with hole drilled to receive needle; turned and soldered into tubing. 10. M inch brass tubing, 0.014 inch wall. 11. Rubber gasket M inch diameter, X inch hole, %42 inch thick. 12. Brass washer M inch diameter, 34 inch hole, Y32 inch thick. 18. "O"' ring Y4 inch o.d., X inch i.d. 14. Packing nut, 3f6 inch threads, with 450 chamfer. 15. "L" fitting with taper ream.